Stable and high capacity Sepharose derivatives for affinity chromatography

High-Capacity Adsorbents for Affinity Chromatography

One simple structural change in compound (I), the destruction of its symmetry by its conversion into n-propyl-2-pyridyl disulphide [III, R = C3H7(propyl)-S-S-2-Py], has permitted the demonstration of an important property of the thiol proteinases, the existence of nucleophilic character in the uninterrupted thiol-imidazole interactive systems of their active centres. The reactions of 2-Py-S-S-2...

Purification of the mammalian hexokinases by affinity chromatography on sepharose-N-aminoacyl-glucosamine derivatives.

DNA gyrase: affinity chromatography on novobiocin-Sepharose and catalytic properties.

Novobiocin-Sepharose was prepared by coupling of novobiocin to Epoxy-activated Sepharose 6B and used as an affinity adsorbent. Four novobiocin-binding proteins were isolated from crude extracts of Escherichia coli with molecular weights of 105, 92, 85 and 40 kdal. The two larger proteins were identified as the A subunit (gyrA protein) and the B subunit (gyrB protein) of DNA gyrase topoisomerase...

Immobilized insulin for high capacity affinity chromatography of insulin receptors.

Insulin receptors can be purified by affinity chromatography on immobilized insulin, but published methods all suffer from a rather low capacity of the affinity columns. By using insulin that has been protected in positions A1 and B29, we have been able to couple the insulin selectively through the B1 amino group to divinyl sulfone-activated agarose. The N terminus of the B-chain is the most in...

Affinity chromatography of viral DNA polymerases on pyran-sepharose.

Pyran covalently linked to cyanogen bromide-activated Sepharose has been shown to be an effective affinity matrix for several viral DNA polymerases. Differential salt elution of viral compared with cellular polymerases, as well as substrate elution, suggests the affinity nature for the matrix. Unlike some other affinity systems described, pyran-Sepharose is totally resistant to nuclease digesti...